51-year-old Kathryn Blount succumbed to her injuries two days after the crash on Cunningham Drive.
Construction on the new Midtown Tunnel connecting Norfolk and Portsmouth was completed months ahead of schedule.
The program allocates $14.4 billion to transportation projects over the next six fiscal years, beginning July 1.
The first drivers officially passed through one lane of the new Midtown Tunnel Friday.
The single-vehicle accident happened just before 12:43 p.m. on I-64E, east of Jefferson around Victory.
I-664 north has been reopened and lanes of traffic are clear.
A car overturned on Dam Neck Road, near London Bridge Road, and caused delays for motorists Wednesday morning.
The Hampton Roads region will receive millions of dollars in transportation improvement over the next six years.
The bus was carrying 25 children. There was one reported minor injury.
The road was closed for several hours after the crash, creating major backups in the area.
The accident happened at George Washington Highway and Alabama Avenue.
Police say the crash involved a Norfolk school bus, a Nissan Sentra and a Lexus.
Portsmouth Fire-Rescue responders are walking through the new Midtown Tunnel Thursday morning.
WAVY Traffic Reporter Jen Lewis is reporting that there were injuries in the crash.
Traffic was diverted to Interstate 264 east. All lanes are now back open and the scene is clear.